The MTS assay is often described as a 'one-step' MTT assay, which offers the convenience of adding the reagent straight to the cell culture without the intermittent steps required in the MTT assay.

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We investigated the applicability and limitations of the MTT assay in representing treatment toxicity, cell viability, and metabolic activity.

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Feb 21, 2026 · The MTT assay, abbreviated “ 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ” assay, is a colorimetric assay frequently used to evaluate cell viability and proliferation.

Actively respiring cells convert the water-soluble MTT to an insoluble purple formazan. The formazan is then solubilized and its concentration determined by optical density.

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